The entrance is on Viale Vaticano, 00165 Rome. Ottaviano and Cipro are the nearby Metro line A stops. You pass a metal detector before entering, including with a booked ticket.
Your shoulders must be covered. Low necklines and shorts above the knee are not allowed. Miniskirts and hats are also banned. The clothing rule applies to the Sistine Chapel too.
The cloakroom is free for bags staff consider too large. If you plan to continue to St Peter's, keep your luggage elsewhere. The Museums ask you not to leave bags with them for that visit.
You can take photos for personal use in the Museums. Flash and selfie sticks are banned, as are tripods. Inside the Sistine Chapel, all photography and filming are forbidden.
Wheelchairs are free while available, with an identity document and a deposit. Staff direct families with strollers to lifts. You cannot eat or drink inside the exhibition halls.